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Artikel #:
44315847
CTAB Extraction Buffer, 125 mL -from Cepham Life Sciences
Artikel #:
44315847
€ 76
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| Item Weight | 0.3 lbs (140 grams) |
OPIS IZDELKA
CTAB Extraction Buffer, 125 mL -from Cepham Life Sciences
About This Item
Introducing CTAB Extraction Buffer, the ultimate solution for extracting genomic DNA from various plant samples. Developed by Cepham Life Sciences, this high-quality buffer is specially formulated to overcome the challenges posed by the rigid cell wall and plant biomolecules such as polysaccharides and polyphenols. When it comes to extracting DNA from plant tissues, the presence of polysaccharides and polyphenols can be a major hindrance. Not only do these compounds vary widely between plant species, but they can also inhibit downstream applications like polymerase chain reaction (PCR).
That's where our CTAB Extraction Buffer comes in. With the help of Polyvinylpyrrolidone, a powerful polyphenol binding agent, our buffer effectively removes polysaccharides and polyphenols, ensuring clean DNA extraction. The extraction protocol involves homogenizing the plant samples in CTAB Extraction Buffer, followed by centrifugation to remove debris and polysaccharides. This step ensures that you obtain a pure DNA extract. One of the key advantages of using our CTAB Extraction Buffer is that it preserves not just DNA, but also RNA.
By simply adding RNase A, you can easily remove RNA from your extract. The DNA is then precipitated and washed in organic solvents to further purify it before re-dissolving it in an aqueous solution. Each bottle of our CTAB Extraction Buffer contains 125 mL of the solution, which is formulated with 2% CTAB, 20 mM EDTA*Na2*2H2O, 1.4 M NaCl, and 100 mM Tris at pH 8. Rigorously tested and validated, our buffer guarantees reliable and consistent results for your research needs. Please note that our CTAB Extraction Buffer is intended for research use only and is not suitable for human or animal clinical/therapeutic use.
To ensure your safety, please follow all safety instructions provided. When it comes to reliable DNA extraction from plants, trust the expertise of Cepham Life Sciences and choose our CTAB Extraction Buffer. Revolutionize your research today by adding this essential tool to your laboratory arsenal.
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Vprašanja in odgovori kupcev
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Vprašanje:
What is CTAB Extraction Buffer used for?
Odgovor: CTAB Extraction Buffer is primarily used for the extraction of nucleic acids from various plant and microbial samples. It contains a cationic detergent that helps lyse cells and neutralize nucleases, ensuring the integrity of the DNA or RNA being extracted. This buffer is particularly effective when working with challenging samples like seeds or plant tissues, which often require additional support to break down cellular structures. -
Vprašanje:
How do I use CTAB Extraction Buffer for DNA extraction?
Odgovor: To use CTAB Extraction Buffer for DNA extraction, mix the buffer with your sample in a tube, then incubate at a controlled temperature to facilitate cell lysis. After incubation, add chloroform to separate the DNA from proteins and other cellular debris. Finally, precipitate the DNA with isopropanol, followed by washing with ethanol. This method is ideal for isolating high-quality DNA necessary for downstream applications such as PCR and sequencing. -
Vprašanje:
Can CTAB Extraction Buffer be used for RNA extraction?
Odgovor: CTAB Extraction Buffer is not typically recommended for RNA extraction, as it can introduce contaminants that inhibit downstream applications. While it can help release RNA from cells, the presence of CTAB may lead to the formation of complexes that degrade RNA quality. For RNA extraction, specific buffers optimized for that purpose are advised to yield high-purity RNA suitable for analysis. -
Vprašanje:
Is CTAB Extraction Buffer suitable for bacterial samples?
Odgovor: Yes, CTAB Extraction Buffer works well for bacterial samples. Its cationic properties assist in lysing bacterial cell walls, allowing for the efficient extraction of DNA. When extracting from bacteria, it is essential to optimize the protocol by adjusting the incubation time and temperature, ensuring that the cell membranes are thoroughly disrupted without excessive degradation of the DNA. -
Vprašanje:
What sample types can I use with CTAB Extraction Buffer?
Odgovor: CTAB Extraction Buffer can be effectively used with various sample types, including plant leaves, seeds, fungi, and certain bacteria. When using this buffer, it is crucial to consider the composition of the sample, as tougher cellular structures may require prolonged incubation for thorough lysis. This versatility makes it a favored choice in molecular biology labs for diverse applications. -
Vprašanje:
How should I store CTAB Extraction Buffer?
Odgovor: CTAB Extraction Buffer should be stored at -20°C to maintain its stability and effectiveness. Ensure the buffer is kept in a tightly sealed container to prevent contamination. Proper storage is crucial because exposure to higher temperatures can degrade the buffer, impacting its performance in nucleic acid extraction processes. Always check the buffer before use for any signs of precipitation or contamination. -
Vprašanje:
What is the shelf life of CTAB Extraction Buffer?
Odgovor: CTAB Extraction Buffer typically has a shelf life of several months to a year when stored properly at -20°C. However, actual shelf life can vary based on storage conditions and the absence of contamination. Users should always perform a visual inspection before use and conduct a control experiment to ensure that the buffer still performs effectively in extracting nucleic acids. -
Vprašanje:
Can I use CTAB Extraction Buffer for QPCR applications?
Odgovor: CTAB Extraction Buffer is not preferred for quantitative PCR (QPCR) applications due to potential impurities and contaminants that could inhibit the PCR reaction. For QPCR, it is crucial to use high-purity DNA prepared using methods specifically designed for quantitative assays. Alternative extraction kits that yield nucleic acids free of contaminants are recommended for QPCR efficiency. -
Vprašanje:
What precautions should I take when using CTAB Extraction Buffer?
Odgovor: While using CTAB Extraction Buffer, wearing gloves and goggles is crucial to avoid skin and eye irritation due to its cationic properties. Additionally, work in a fume hood or well-ventilated area when handling this buffer, particularly during the chloroform extraction step, as it can release harmful vapors. Ensuring meticulous pipetting and using sterile equipment helps minimize the risk of contamination in your experiments. -
Vprašanje:
Where can I buy CTAB Extraction Buffer, 125 mL - from Cepham Life Sciences in Slovenia?
Odgovor: You can purchase CTAB Extraction Buffer, 125 mL from Cepham Life Sciences through Ubuy. Ubuy offers a convenient online platform with a variety of products, making it easy to find and procure specialized scientific reagents like the CTAB Extraction Buffer. Visit Ubuy's website and search for the product to explore the options available in Slovenia.
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Prednosti
- High purity
- Reliable results
- Ease of use
- consistent performance
- Suitable for various applications
Product Price History
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