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Recommended for resolution of RNA and DNA fragments larger than 1500 bp.
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Podrobnosti produkta
| Item Weight | 2 lbs (910 grams) |
OPIS IZDELKA
50x TAE Buffer (1 L)
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Vprašanja in odgovori kupcev
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Vprašanje:
What is TAE Buffer and its primary applications?
Odgovor: TAE Buffer (Tris-Acetate-EDTA) is a commonly used buffer in molecular biology, primarily for DNA electrophoresis. It helps maintain a stable pH during the separation process, ensuring the integrity of nucleic acids. Researchers utilize TAE Buffer for applications like gel electrophoresis and PCR, where precise pH control is vital to obtaining reproducible results and enhancing DNA band resolution. -
Vprašanje:
How should TAE Buffer be prepared or diluted for use?
Odgovor: To prepare TAE Buffer, mix the appropriate amounts of Tris, acetic acid, and EDTA in distilled water. For electrophoresis, the 1X working solution is typically used, which can be made by diluting the 50X stock solution. Following the manufacturer's instructions for preparation ensures optimal results, which is crucial for successful DNA analysis and research applications. -
Vprašanje:
Can I use TAE Buffer for RNA electrophoresis?
Odgovor: While TAE Buffer is predominantly used for DNA electrophoresis, it can also be used for RNA. The buffer helps maintain the RNA's stability during gel electrophoresis, although some researchers prefer using TBE Buffer for RNA due to its lower ionic strength. Depending on your experiment, TAE can be a suitable choice, especially if DNA and RNA analysis are both being conducted. -
Vprašanje:
What are the benefits of using a 50X TAE Buffer concentrate?
Odgovor: A 50X TAE Buffer concentrate is beneficial for saving storage space and allowing flexibility in dilutions. Researchers can easily prepare various working concentrations based on their specific experimental needs. This concentrated form also provides long shelf life when stored properly, making it convenient for laboratories that frequently conduct DNA analysis. -
Vprašanje:
Is TAE Buffer suitable for southern blotting techniques?
Odgovor: Yes, TAE Buffer can be utilized in southern blotting techniques. It aids in the separation and transfer of DNA fragments onto a membrane, which is essential for further processing and analysis such as hybridization. Researchers often prefer using TAE due to its ability to maintain the integrity of the DNA during the blotting process. -
Vprašanje:
How does TAE Buffer compare to TBE Buffer?
Odgovor: TAE Buffer and TBE Buffer both serve as buffers during electrophoresis but have different ionic strengths and properties. TAE Buffer is generally used for DNA analysis, while TBE Buffer is preferred for high-performance applications. Choosing between them depends on specific experimental requirements, such as the type of nucleic acid and the resolution needs of your gel. -
Vprašanje:
What is the role of EDTA in TAE Buffer?
Odgovor: EDTA (Ethylenediaminetetraacetic acid) in TAE Buffer chelates divalent cations like magnesium and calcium, which can inhibit enzymatic reactions. This is particularly important if you are working with nucleases that could degrade DNA. Maintaining a low concentration of these ions helps protect your DNA samples during experiments. -
Vprašanje:
Can TAE Buffer be reused after electrophoresis?
Odgovor: While it is technically possible to reuse TAE Buffer, it is generally not recommended due to potential contamination from nucleic acids and proteins that may have migrated during electrophoresis. Reusing the buffer can lead to inconsistent results, so preparing fresh buffer for every experiment is advisable for maintaining experimental integrity. -
Vprašanje:
What storage conditions are recommended for TAE Buffer?
Odgovor: TAE Buffer should be stored in a cool, dark place to prevent degradation. Ideally, it should be kept at room temperature and tightly sealed to avoid contamination. This helps in maintaining its effectiveness for prolonged periods, allowing researchers to rely on its consistency throughout their experiments. -
Vprašanje:
Where can I buy 50x TAE Buffer (1 L) in Slovenia?
Odgovor: You can buy 50x TAE Buffer (1 L) from Ubuy, which offers a wide range of laboratory supplies and reagents. The platform provides various options that cater to the needs of researchers and labs looking for quality products, making it a dependable source for your laboratory needs.
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Prednosti
- High purity for reliable results
- Convenient 1 L packaging
- consistent performance in electrophoresis
- Ready-to-use solution saves time
- Excellent for DNA/RNA analysis
Slabosti
- Occasional sediment formation
Product Price History
Pomembne informacije
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Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
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Značilnosti in prednosti
- 50X TAE Buffer is essential for nucleic acids electrophoresis.
- Suitable for agarose and polyacrylamide gel applications.
- Effective as both a running buffer and gel preparation buffer.
- Filtered through a 0.22 µm membrane for optimal purity.
- Ideal for genomic and large supercoiled DNA.
- Perfect for resolving RNA and DNA fragments over 1500 bp.