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Artikel #:
86739675
5M Ammonium Acetate Buffer Solution, 250 mL
Artikel #:
86739675
€ 68
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Naročite zdaj in dobite okoli dne: Torek, Julij 07
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Our 5M Ammonium acetate solution is prepared in molecular biology grade/ultrapure water, filter sterilized with 0.22 µm filter and DNase/RNase/Protease Free.
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Podrobnosti produkta
| Item Weight | 0.55 lbs (250 grams) |
OPIS IZDELKA
5M Ammonium Acetate Buffer Solution, 250 mL
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Vprašanje:
What is 5M Ammonium Acetate Buffer Solution used for?
Odgovor: 5M Ammonium Acetate Buffer Solution is primarily utilized in molecular biology, biochemistry, and analytical chemistry as a buffering agent. It helps maintain a stable pH during various experiments, particularly in nucleic acid isolation and protein purification. This stability is crucial for minimizing enzymatic degradation, ensuring authenticity in results. For instance, if you are conducting gel electrophoresis, using this buffer can help in maintaining optimal pH levels, thus enhancing the resolution of your sample separates. -
Vprašanje:
How should I store 5M Ammonium Acetate Buffer Solution?
Odgovor: 5M Ammonium Acetate Buffer Solution should be stored in a cool, dark place, ideally at temperatures between 2-8°C. It’s important to keep it tightly sealed to prevent contamination and evaporation of the buffer components. If stored correctly, the solution retains its effectiveness for a long time. Scientists often keep it in lab fridges alongside other reagents to ensure they have quick access when conducting experiments such as PCR or gel electrophoresis. -
Vprašanje:
Is the 5M Ammonium Acetate Buffer Solution suitable for all types of experiments?
Odgovor: While versatile, 5M Ammonium Acetate Buffer Solution may not be suitable for every type of experiment. Its specific pH range and ionic strength make it ideal for certain DNA and RNA applications, but not for assays sensitive to specific buffer ions. Therefore, it’s essential to consider the requirements of your particular protocol. For instance, it’s commonly used in DNA extraction but might not be recommended for enzyme reactions that require pure water conditions. -
Vprašanje:
What safety precautions should I take when using 5M Ammonium Acetate Buffer Solution?
Odgovor: When using 5M Ammonium Acetate Buffer Solution, always wear appropriate personal protective equipment such as gloves, goggles, and lab coats. Although it is generally considered safe, it can be irritating to skin and eyes. It's crucial to work in a well-ventilated area and to understand the Safety Data Sheet (SDS) for handling and disposal. For example, if spills occur, protocols for neutralizing and cleaning should be in place to minimize exposure and environmental impact. -
Vprašanje:
Can I dilute 5M Ammonium Acetate Buffer Solution for other applications?
Odgovor: Yes, 5M Ammonium Acetate Buffer Solution can be diluted to suit various applications. Depending on your experimental needs, you can prepare lower concentrations, such as 1M or 0.5M, by mixing with deionized water. This flexibility allows researchers to tailor conditions based on specific protocols, such as preparing reaction buffers or sample diluents. Always ensure that you calculate dilution ratios accurately to maintain effective concentrations. -
Vprašanje:
What are the common applications of Ammonium Acetate Buffer Solution?
Odgovor: Common applications of 5M Ammonium Acetate Buffer Solution include DNA and RNA extraction, protein purification, and chromatography. Its buffering capacity is particularly useful in maintaining pH stability during these processes. For example, in RNA isolation, using the correct buffer ensures that the nucleic acids remain intact and are not degraded. Similarly, in protein studies, this solution helps maintain functional and structural integrity during analyses. -
Vprašanje:
Is 5M Ammonium Acetate Buffer Solution compatible with other reagents?
Odgovor: 5M Ammonium Acetate Buffer Solution is compatible with many biological reagents, making it a versatile component in many procedures. However, it’s important to check compatibility with specific enzymes or substrates, as some may be sensitive to ammonium ions. For example, it works well with DNases and RNases, enhancing their activity in molecular biology applications. However, always perform preliminary tests when using it alongside new reagents. -
Vprašanje:
Can 5M Ammonium Acetate Buffer Solution be used in protein crystallization?
Odgovor: Yes, 5M Ammonium Acetate Buffer Solution is often used in protein crystallization experiments. Its buffering properties help maintain a stable pH which is critical during crystallization trials. This stability aids in achieving higher-quality crystal formations. Researchers often assess various buffer conditions, including ammonium acetate, to optimize crystallization setups that can lead to better structural insights of proteins. -
Vprašanje:
How does 5M Ammonium Acetate Buffer Solution compare to other buffer systems?
Odgovor: 5M Ammonium Acetate Buffer Solution offers distinct advantages over other buffer systems, particularly in its simplicity and effectiveness at maintaining pH. Unlike phosphate buffers, it minimizes precipitation issues in ionic interactions. This makes it ideal for DNA and RNA manipulation. Researchers may choose ammonium acetate buffers over others in applications like gel electrophoresis for sharper band resolution and better sample integrity. -
Vprašanje:
Where can I buy 5M Ammonium Acetate Buffer Solution, 250 mL in Slovenia?
Odgovor: You can buy the 5M Ammonium Acetate Buffer Solution, 250 mL from Ubuy in Slovenia. Ubuy provides a convenient online shopping experience, allowing you to browse a selection of laboratory chemicals and reagents to meet your research needs. You will find detailed product information and the option to procure the buffer solution with assurance of quality.
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€ 68
Naročite zdaj in dobite okoli dne: Torek, Julij 07
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Secured transaction
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
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Značilnosti in prednosti
- 5M Ammonium acetate solution
- Prepared in molecular biology grade/ultrapure water
- Filter sterilized with 0.22 µm filter
- DNase/RNase/Protease Free
- Used in DNA and RNA purification, biological buffers, chemical analysis, in pharmaceuticals, and in preserving foods